TSS: scRNA-seq with Parse Biosciences Evercode Single Cell Whole Transcriptome Barcoding Kits

Seminar Abstract: The Evercode split pool combinatorial barcoding platform by ‪@parsebio‬ has enabled researchers to perform single cell experiments with unprecedented scale and ease. The ability to scale up to 1 million cells or nuclei across up to 96 samples at a time means you no longer need to compromise on experimental design. The Evercode platform is entirely consumable based, there is no custom equipment, just a set of regents. The kits include everything needed to bring samples from a single cell suspension to sequencing ready molecules. The barcoding and library preparation portion of our assay leverages split-pool combinatorial barcoding enabling the profiling of single-cell transcriptomes without microfluidic instruments. The Parse Biosciences assay leverages the cell itself as its own compartment to perform multiple rounds of splitting, pooling, and ligation of transcripts to generate different barcode combinations unique to each cell’s set of transcripts. Four rounds of barcoding can yield 14,155,776 possible barcode combinations (three rounds of barcoding in 96x96x96 wells followed by a fourth round with 16 PCR reactions), enough to uniquely label up to one million cells while avoiding doublets. This approach is robust, flexible, and economical. The first step of the workflow is fixation, after which samples can be stored -80c until ready for barcoding. This facilitates the flexibility to collect multiple samples over several days, allowing for time-course based analysis. Listen in for more details! Chapters: 0:00 – Welcome 0:43 – Introduction to the Parse Biosciences Team 1:43 – About Parse Biosciences 3:28 – Introducing Evercode Whole Transcriptome Barcoding Kits 7:04 – Conventional scRNA-seq vs. Evercode 9:15 – Evercode Split Pool Combinatorial Barcoding 13:24 – Measuring Doublet Rates 15:28 – Case Studies 18:11 – Improvements in Gene Detection 21:00 – Optimize Your Sequencing 22:06 – Workflow Flexibility Enabled Through Fixation 24:34 – Evercode Library Prep 26:26 – Computational Pipeline and Analysis 28:06 – Customer Training Programs 28:43 – Future Applications 32:37 – Q&A Learn more about Parse Biosciences: Evercode Combinatorial Barcoding Technology – https://www.parsebiosciences.com/tech... Evercode WT Mini Kit – https://www.parsebiosciences.com/prod... Evercode WT Kit – https://www.parsebiosciences.com/prod... WT Mega Kit – https://www.parsebiosciences.com/prod... -------------------------------------------------- For more information on upcoming MaGIC seminars: https://bioinformagic.io/events Follow MaGIC for more content: Twitter:   / magic_analytics   Instagram:   / magic_analytics   #bioinformatics #genomics #parsebiosciences #parsebio #singlecell #proteomics #sequencing #evercode #scRNAseq