TSS: Sequencing of anything, by anyone, anywhere, at any read length with Oxford Nanopore

Seminar Abstract: Join us for an overview of ‪@OxfordNanoporeTechnologies‬' sequencing tools, applications, and recent advancements. Chemistry Overview – R10.4.1 flow cells with the latest Kit14 chemistry deliver high-accuracy, high-output sequencing data, achieving 99.6% single molecule raw-read simplex accuracy and 99.92% duplex accuracy. The newer chemistry offers tunable run conditions, providing more flexibility between accuracy and output. Methylation analysis – Study methylation without bisulfite conversion. Bioinformatics tools, such as Remora, provide a comprehensive workflow for characterizing methylation. Short Fragment Mode – Sequence fragments as short as 20bp and study the nucleosome of cell-free DNA. Adaptive Sampling – Enrich or deplete regions of interest at the software level—no need to enrich for your target upstream of sequencing. Application Focus – Single-cell transcriptomics using 10X libraries; Inexpensive rapid microbial whole-genome; Full-length plasmid sequencing without tiling; Immunogenetics; Native RNA sequencing to explore RNA base modifications; Bulk RNA-seq for analyzing gene expression, full-length isoform, and alternative splicing. Chapters: 0:00 – Welcome and Introduction to the Oxford Nanopore Team 2:08 – Nanopore DNA/RNA Sequencing 8:13 – Methylation, WGS & Targeted Sequencing 21:40 – RNA Research 28:09 – AAV/Plasmids 39:36 – Microbial & Metagenomics 47:47 – Summary 50:22 – Q&A To learn more about Oxford Nanopore: Flongle – https://nanoporetech.com/products/flo... MinION – https://nanoporetech.com/products/minion GridION – https://nanoporetech.com/products/gri... PromethION 2 – https://nanoporetech.com/products/pro... -------------------------------------------------- For more information on upcoming MaGIC seminars: https://bioinformagic.io/events Follow MaGIC for more content: Twitter:   / magic_analytics   Instagram:   / magic_analytics   #bioinformatics #genomics #oxfordnanopore #longreadsequencing