How long does it take for a column to equilibrate?

There is an exact answer. You don’t need to guess. It takes 5 column volumes to equilibrate a column. Now, you ask yourself the question, “What is a column volume?” That’s how long it takes to flush the column one time. That’s your t0. Read the blog here: https://axionlabs.com/chromatography-... 🔗 Follow Us: Website: https://axionlabs.com/ Facebook:   / axionlabs   LinkedIn:   / axion-labs   #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our 5 day hands-on LC/GC bootcamp. If you can't make it to Chicago choose one of our online courses. After taking the LC/GC bootcamp further advance your skills by taking the advanced topics courses.