Clean a reverse phase HPLC Column in 2 steps.

Cleaning a reverse-phase HPLC column doesn’t have to be complicated. In this step-by-step guide, Dr. Lee Polite explains how to flush out salts, buffers, and sticky non-polar residues using simple solvent changes. You’ll learn exactly when to use water-rich mobile phase, when to switch to 100% organic, and how to keep your columns running longer. Prefer to read: https://axionlabs.com/chromatography-... 👉 Related Videos: How to Clean a C18 Column – extra details on cleaning a dirty or clogged frit https://axionlabs.com/chromatography-... HPLC Demystified Part 1 – a refresher on why separation depends so much on polarity    • Do I need to use an HPLC Guard Column?   How to test an HPLC column -check efficiency and decide whether cleaning helped https://axionlabs.com/chromatography-... 📌 Topics Covered: Why columns get dirty (salts, buffers, sample oils) Step 1: Water flush to remove polar contaminants Step 2: Organic flush to remove non-polar residues Shortened cleaning protocol if you’re in a rush What cleaning can’t fix (physical column damage) 🎓 Want hands-on training? Join us at Axion Labs in Chicago for 5-day chromatography bootcamps in HPLC and GC. https://axionlabs.com/lc-gc-bootcamp/ Hashtags: #HPLC #Chromatography #LabTips #HPLCTraining #ReversePhase #AnalyticalChemistry #HPLCColumns