factors affecting column equilibration

Is column equilibration a waste of time? To a few a column equilibration may appear a waste of time but it is a necessary step to generate reliable chromatographic data. The inadequate column equilibration can result in baseline disturbance, retention time variation, or even response variation. Let us explore the factors that can influence the column equilibration process… Join the HPLC Method Development WhatsApp group of Pharma Growth Hub to receive more updates: https://chat.whatsapp.com/GyOUBdaVqGR... Regards Bhaskar #column #hplc #chromatography #PharmaGrowthHub