Vincristine & Vinblastine (Part 01) = Industrial Production, Estimation and Utilization (Part 17)

Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test, and other GPAT Materials - https://play.google.com/store/apps/de... Vincristine and Vinblastine Vincristine and Vinblastine are isolated from the dried entire plant of Catharanthus roseus Linn (Apocynaceae). Common Names Madagascar periwinkle, Bigleaf Periwinkle, Large Periwinkle, Periwinkle, Vinca (formerly known as Vinca rosea). Extraction and Isolation The dried leaf material is taken and is extracted with a solution of hot ethanol-water–acetic acid in a ratio of 9:1:1. The solvent is removed and to the residue, a hot hydrochloric acid solution of 2% is added. The pH of the acidic extract is adjusted to 4, for the precipitation of the non-alkaloidal components, which can be separated by filtration. The pH of the aqueous acidic solution is now adjusted to 7 and then extracted with benzene. The benzene layer is evaporated to obtain vinblastine and other alkaloids. Isolation of Vinblastine and Vincristine The phenolic materials are removed by washing the extract with dilute alkali. The washed extract is subjected to chromatography on alumina and elution is carried out in 18 fractions starting with benzene–methylene chloride (65:35) mixture to pure methylene chloride. Vinblastine recovered in the ninth fraction. Further elution of the column results in separating the fractions of vincristine. Melting point: Vinblastine: 284–285°C, Vincristine: 273–281°C Thin Layer Chromatography of Vincristine Vincristine dissolved in 25% water in methanol solution, spotted in Silica gel-G plate and developed using the solvent, acetonitrile: benzene (30:70). The dried plates are sprayed with 1% solution of ceric ammonium sulfate in 85% phosphoric acid. The Rf value of the appeared spot would be 0.39. Uses Vinblastin is an antitumor alkaloid used in the treatment of Hodgkin’s disease. Vincristine is a cytotoxic compound used to treat leukemia in children. Get in touch with the solution by just clicking the following links- Facebook Group-   / solutionpharamcy   Mobile App - https://play.google.com/store/apps/de... New Channel (Pharmacy Dictionary)    / @pharmacydictionary   E-Mail for official and other work - [email protected] #solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPAT