MoveTip #5: Use free software to get the most out of your LCMS Separation

⚡ Brief Summary When translating a LC-MS method you have seen published or developing a new one, knowing the optimal flow rate and the appropriate pressure is typically an unanswered question. Never fear, a good estimate can be calculated! In this MoveTip, we share an easy method using a tool called “Capillary Flow Calculator” within the PNNL Molecular Weight Calculator, to estimate column pressure, optimal flow rate, and post-column band broadening. 🧪 What You’ll Learn to try It Yourself When using or developing a new LCMS method, you’ll learn how to: • Where to download the PNNL Molecular Weight Calculator application • Make a very good estimate of the pressure the column should be operating • Estimate the flow rate where the column may perform best for your separation 🧠 About MoveTips MoveTips is a short-format educational series from Move Analytical — practical hacks, features, and insights to make metabolomics easy and reproducible. We do a lot of lab work ourselves, so we post new videos frequently on topics such as Skyline, sample prep, and various laboratory workflows which we hope you will find helpful, whether you’re new to metabolomics or a seasoned pro. 🔗 Learn More Move Analytical Website: https://www.moveanalytical.com/?utm_s... Download the PNNL Molecular Weight Calculator tool: https://pnnl-comp-mass-spec.github.io... 💬 Join the Discussion What is your own favorite metabolomics tip or mass spec laboratory hack? Share it below — we might feature it in our next MoveTip! 👍 Like, 🔔 Subscribe, and share if this helped your workflow. #MoveTips #Skyline #Metabolomics #MassSpectrometry #MoveAnalytical #labhack