Who's Really There? Long-Read 16S and Adaptive Amplification Reveal Microbiomes You've Been Missing
Most 16S sequencing workflows have a dirty secret: what you see in your data isn't always what's actually in your sample. Fixed-cycle PCR introduces artifacts, distorts community profiles, and amplifies noise. Short hypervariable regions leave entire taxonomic groups hiding in plain sight. The result is a microbiome that looks complete but isn't. In this webinar, researchers from USDA-ARS and scientists from PacBio and n6 share how combining full-length 16S long-read sequencing with adaptive, per-well amplification control is fundamentally changing what's discoverable in complex microbial communities. You'll see real-world data showing how autonormalized library preparation more than doubles unique ASV detection on PacBio, jumping from 24% to 59% of reads mapping to unique ASVs, while delivering balanced read counts across sample inputs spanning orders of magnitude in concentration. From insect gut microbiomes to agricultural soils, the organisms you've been missing are ready to be found. 0:00 Introduction 0:56 Jeremy E. Wilkinson, PhD 9:50 Mary Arrastia, PhD 20:10 Charles Mason, PhD 42:15 Q&A - - - - - - - - - - - - - - - - - - - - ABOUT SAMBA SCIENTIFIC Samba Scientific is a leading life science and biotech marketing agency specializing in innovative and data-driven marketing services tailored to the life sciences and biotechnology sectors. Our team of experts understands the industry's unique challenges and delivers strategic solutions that drive engagement, growth, and brand visibility. Whether launching a new product, expanding your reach, or enhancing your digital presence, Samba Scientific provides the expertise and creativity to help you succeed. VISIT OUR WEBSITE https://sambasci.com CONNECT WITH US ON SOCIAL MEDIA LinkedIn: / sambascientific Instagram: / sambascientific Facebook: / sambascientific X: / sambasci CONTACT US For inquiries and more information, reach out to [email protected] Don't forget to subscribe for more life science marketing insights and updates! #Biotech #LifeSciences #Pharma #Marketing #LifeScienceMarketing #BiotechMarketing #MarketingAgency #MarketingServices #SambaScientific #STEM #Biology #Science
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