Bioanalytical Chemistry: Antibodies as reagents

Note: Typo at 07:29 -- molecular weight of a single Fab fragment is 50 kDa, not 25 kDa. Made for CHEM 5720, Methods in Bioanalytical Chemistry Prof. Rebecca R. Pompano, PhD University of Virginia Department of Chemistry Spring 2022 This video lesson introduces antibodies from the perspective of a bioanalytical chemist. Learning goals: 1. Know what is an antibody and antigen 2. Draw the structure of an antibody and be able to name its component parts and fragments 3. Know the molecular weight of each region of the antibody 4. Describe how polyclonal and monoclonal antibodies are produced 5. Define antibody clonality and what it means for binding sites on the antigen 6. List and describe the most common isotypes used in analytical assays 7. List and describe the key parameters of antibody reagents 8. Know why antibodies must be validated 9. Identify storage and handling conditions for antibody reagents