Bioanalytical Chemistry: Kinetics of Antibody Binding

Made for CHEM 5720, Methods in Bioanalytical Chemistry Prof. Rebecca R. Pompano, PhD University of Virginia Department of Chemistry Spring 2020 - COVID19 remote course edition This video lesson introduces the meaning and typical values of rate constants for antibody-antigen binding; how to calculate the longevity of a bound complex, and a brief intro to how rate constants are measured experimentally. Learning goals: 1. Draw a plot of binding over time for a receptor-ligand pair. 2. List typical rate constants for antibody-antigen association and dissociation 3. Calculate the half-life of an antibody-antigen complex 4. Describe the basics of how k-a (k-on) and k-d (k-off) are measured experimentally