Bioanalytical Chemistry: Validation & Troubleshooting of Immunoassays

Made for CHEM 5720, Methods in Bioanalytical Chemistry Prof. Rebecca R. Pompano, PhD University of Virginia Department of Chemistry Spring 2020 - COVID19 remote course edition This video lesson introduces validation and troubleshooting of immunoassays for complex biological samples: Figures of merit Impact of matrix effects & nonspecific binding Impact of cross-reactivity of antibodies Troubleshooting for calibration curves: high background, low signal, noise