PyMOL Tutorial: Active Site Modeling

As part of our JoVE manuscript on biomolecular modeling protocols, we created supplemental videos detailing the protocol for each program. Enjoy this PyMOL tutorial, featuring the active site of glucokinase. REFERENCES A supporting video for our JoVE manuscript, available now at: https://www.jove.com/t/63170/modeling... • Procko, K., Bakheet, S., Beckham, J. T., Franzen, M. A., Jakubowski, H., & Novak, W. R. (2021). Modeling an Enzyme Active Site using Molecular Visualization Freeware. Journal of visualized experiments: JoVE, (178). • PDB ID 3FGU: https://www.rcsb.org/structure/3FGU • Petit, Pierre, Mathias Antoine, Gilles Ferry, Jean A. Boutin, Amandine Lagarde, Laure Gluais, Renaud Vincentelli, and Laurent Vuillard. "The active conformation of human glucokinase is not altered by allosteric activators." Acta Crystallographica Section D: Biological Crystallography 67, no. 11 (2011): 929-935. • Wang J, Youkharibache P, Zhang D, Lanczycki CJ, Geer RC, Madej T, Phan L, Ward M, Lu S, Marchler GH, Wang Y, Bryant SH, Geer LY, Marchler-Bauer A. iCn3D, a web-based 3D viewer for sharing 1D/2D/3D representations of biomolecular structures. Bioinformatics. 2020 Jan 1;36(1):131-135. doi: 10.1093/bioinformatics/btz502. PMID: 31218344; PMCID: PMC6956771. • https://www.ncbi.nlm.nih.gov/Structur...