Coomassie Purity Staining of Recombinant Antibodies - Addgene Protocol

Looking for a simple, fast, and cheap way to check an antibody’s purity? Well, you’ve found it, with Addgene’s protocol for Coomassie Purity Staining of Recombinant Antibodies! Join Amrita Rhoads, a Lab Tech II at Addgene, as she walks you through this simple, time-saving, in-expensive process. For the full written protocol, head to https://www.addgene.org/protocols/coo... ___________________________________________________________________________ 00:00 - Intro 01:04 - Materials & Reagents 02:25 - Preparation 03:49 - SDS Page 06:41 - Staining the Gel 08:26 - Image Analysis 12:37 - Outro ___________________________________________________________________________ Featuring: Amrita Rhoads and Ashley Waldron Written by: Rachel Leeson & Quintin Marcelino (based on a protocol by Angela Holmes) Directed by: Quintin Marcelino Assistant Director: Anna Kamens Designs by: Jason Snair Edited by: Anna Kamens & Quintin Marcelino Animations by: Quintin Marcelino Special Appearance by: Webster Kiwi Kitty Music and Sound Effects courtesy of Epidemic Sound