Recombinant Antibody Affinity Purification with Protein A or Protein G Protocol

Do you really know what's in your protein supernatant? If not, it sounds like you’re looking to do some Antibody Purification! Purification lets you isolate the antibodies from the other proteins in the supernatant, leaving you an accurate measurements and sparkling clean calculations. Join Amrita Rhoads, a Lab Tech at Addgene, as she shows you just how easy it is to become a purifying pro! For the written protocol, head to: https://www.addgene.org/protocols/aff... ___________________________________________________________________________ Intro - 00:00 Materials - 01:46 Transfection Info - 03:07 Prep - 03:28 Affinity Chromatography - 03:56 Buffer Exchange - 07:12 Storage - 10:11 Next Steps - 10:33 Outro - 10:52 ___________________________________________________________________________ Credits: Featuring: Amrita Rhoads Written By: Rachel Leeson, & Quintin Marcelino Directed, Animated, & Edited by: Quintin Marcelino Videography by: Quintin Marcelino Sound Design by: Quintin Marcelino Designs by: Jason Snair Additional Designs by: Quintin Marcelino Music and Sound Effects courtesy of Epidemic Sound. Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational purposes only. If you choose to reuse or repurpose this SOP in another location, please note that you do so at your own risk; you should ensure that any local guidance is also adhered to. None of the authors, contributors, administrators, or anyone else associated with Addgene, can be held responsible for your use of the information contained in or linked to from these web pages.