How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells Cultured in mTeSR™ Plus

In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1, into mTeSR™ Plus. This video will cover the generation of cell aggregates and describe a passaging protocol using ReLeSR™ or gentle cell dissociation reagent (GCDR). 0:00 - Intro 1:23 - ReLeSR™ Passaging 3:14 - GCDR Passaging 5:56 - Plate Setup for mTeSR™ Plus Transition Key topics covered in this video: Assessing quality of hPSC cultures through morphology using phase-contrast microscopy to determine when to passage Passaging cell aggregates using ReLeSR™ or GCDR Optimizing aggregate size for feeder-free PSC culture Plating aggregates into maintenance medium Watch the other two videos in the series below: Video 1: Plate Coating for hPSC Cultures -    • How to Coat Plates for Human Pluripotent S...   Video 3: Maintenance and Morphological Assessment -    • How to Maintain and Assess Morphology of H...   Learn more about mTeSR™ Plus hPSC maintenance media: https://www.stemcell.com/why-mtesrplus View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus: https://www.stemcell.com/transitionin... Learn more about passaging without scraping with ReLeSR™: https://www.stemcell.com/relesr.html #hPSCculture #HumanPluripotentStemCells #mTeSRPlus For a full list of products, as well as educational resources, visit our website: https://www.stemcell.com Explore STEMCELL’s official social media channels and follow us on your favorite platforms: https://www.stemcell.com/social-media