Immunohistochemistry
In this detailed tutorial, we'll walk you through the complete Immunohistochemical protocol, a laboratory technique used to visualize proteinn expression in tissue sections. Whether you're a beginner or just need a refresher, this video covers everything you need to know to successfully perform IHC in your lab. In this video, we cover: 1. Paraffin Removal and Rehydration 2. Antigen Retrieval 3. Quenching and Blocking 4. Antibody Incubation 5. Chromogenic Signal Detection 6. Hematoxylin Counterstaining 7. Dehydration and Mounting 00:00 Introduction to Immunohistochemistry (IHC) 00:25 Paraffin Removal and Rehydration 01:20 Antigen Retrieval 02:34 Quenching and Blocking 04:17 Primary Antibody Incubation 05:16 Chromogenic Signal Detection 06:17 Hematoxylin Counterstaining 07:30 Dehydration and Mounting Follow us on social media: Instagram: / proteintech TikTok: / ptglab LinkedIn: / proteintech-group Bluesky: https://bsky.app/profile/proteintech.... Facebook: / proteintech X: https://x.com/proteintech
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Immunohistochemistry Protocol for Paraffin embedded Tissue Sections
