Cryo EM: Back to Basics - Chapter 3
Join NIS CSO, Giovanna Scapin for the third and final chapter of our webinar series, "Cryo EM: Back to Basics". Hear from Giovanna about challenges in cryo-EM, how to overcome them, and our outlook for the future of this structure determination technique. Catch the previous chapters: Chapter 1: Sample Prep & Imaging | • Cryo-EM: Back to Basics - Chapter 1 Chapter 2: Data Processing & Interpretation | • Cryo-EM: Back to Basics - Chapter 2 Chapters 00:00 Speaker Introduction 01:20 Webinar Introduction 02:48 Cryo-EM Bottlenecks 04:00 Grid Preparation & Screeing 06:09 Data Collection Time 08:45 How Much Data? 12:17 Storage & Computing Time 13:05 Dara Processing & 3D Reconstruction 14:39 Grid Screening & Data Collection 15:35 Sample Requirements 17:55 Sample & Grid Preparation 19:15 Sample Size & Flexibility 21:37 AWI Effects 24:38 Bottlenecks 26:47 Takeaway Messages 28:08 Q&A Session
Cryo-EM: Back to Basics - Chapter 1
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