Biotechnology 007: Yeast Plasmids: Prof. Beckmann

A lecture on Yeast Biology and Plasmids. Time Stamps 0:24 — Yeast is a model eukaryote 6:57 — Selection in yeast 10:22 — Strains 12:34 — yeastgenome.org 13:47 — Lab strains and auxotrophic selection 19:24 — How these strains are made 24:10 — Drop out media 26:02 — Looking at pYES2 30:16 — Types of origins for yeast plasmids 32:51 — Back to pYES2 34:56 — Integration 39:58 — Counter selection 42:41 — Holliday junctions 45:36 — E. coli also have strains 45:57 — The paper lists out mutations 47:56 — Creation of generic yeast plasmids 49:20 — Gal1 Promotor Reading Assignment: 1) Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications https://pubmed.ncbi.nlm.nih.gov/9483801/ 2) Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. https://www.ncbi.nlm.nih.gov/pmc/arti... 3) https://blog.addgene.org/plasmids-101... Join the Discussion on Discord server below:   / discord