Yeast Transformation (Lab Tutorial)

Name: Yeast Transformation (Lab Tutorial)
Uploaded: 2021-03-19T00:00:00.000Z
Description: Transformation is a fundamental skill for any genetics lab. Real samples and research progress towards my biochem PhD degree at uOttawa. Catch some casual experiments live at twitc

Transformation is a fundamental skill for any genetics lab. Real samples and research progress towards my biochem PhD degree at uOttawa. Catch some casual experiments live at twitch.tv/qwedlife 0:00 Intro 0:52 Day 1 - Thawing strains 1:41 Day 2 - Inoculation 2:30 Day 3 - Transformation (OD & Harvest) 4:47 Day 3 - Transformation (Reaction mix) 7:40 Day 3 - Transformation (Heat shock) 8:39 Day 3 - Transformation (Removing PEG & Plating) 11:13 Day 4 - Replica plating 11:54 Day 5 - Patch positives 12:43 Day 6 - Freeze / Test strains

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