TP1-3 Biochimie : Détermination de la vitesse initiale d'une réaction enzymatique

The Enzymology and Metabolic Biochemistry PDF module SVI S4. Bachelor's Degree in Life, Earth, and Universe Sciences. There are courses, summaries, tutorials, practical exercises, multiple-choice questions, exercises, solved exams, free books, and more... You can study with us online and take multiple-choice questions on our forum. You can also download PDF files and study offline. In any case, you must return to this main page for easy navigation. Enzymology 1- Definition and Classification of Enzymes 2- Roles of Enzymes a- The Active Site; Enzymatic Catalysis and the Different Types of Catalysis b- Properties of Enzymes: Specificity and Efficiency 3- Enzymatic Kinetics a- First-Order Kinetics (Rate, etc.) b- Influence of Substrate Concentration on Enzymatic Activity 4- Enzymatic Effectors a. Inhibitors b. Activators 5- Physicochemical Parameters a. Temperature b. pH Metabolic Biochemistry Content: Introduction and definition of metabolism Bioenergetics Definition, Free Energy G, Enthalpy, Entropy, Bonds with high hydrolytic potential. Redox Reactions and Redox Potential. Concept of coupled reaction and common intermediate. Main molecules involved in redox reactions: electron carriers Carbohydrate Metabolism Glycolysis a. Aerobic: Krebs cycle, b. Anaerobic: fermentation) – Pentose phosphate pathway – Mechanism of oxidative phosphorylation – Regulation of glycolysis Lipid metabolism Lipid biosynthesis Lipid degradation Amino acid metabolism Concepts of amino acids Amino acid degradation Amino acid biosynthesis Other metabolic pathways (orogenesis, etc.) Introduction In biochemistry, enzymology studies enzymes and fermentation. Several more specific areas can be identified: the study of enzymatic reactions, including reaction kinetics, the structural approach to enzymes and their relationship to their activity, which also allows enzymes to be classified according to the EC nomenclature. Fermentation, as well as anaerobic respiration, are thought to be older processes than aerobic respiration. The initially low oxygen levels on the planet prompted anaerobic mechanisms in the first organisms. Eukaryotes evolved toward aerobic respiration by developing the ability to use oxygen produced by photosynthesis. Biochemistry is the study of the chemical reactions that take place within living things, particularly within cells. The complexity of biological chemical processes is controlled through cellular signaling and energy transfers during metabolism.