How to Simulate In-Fusion Cloning in SnapGene

In-Fusion cloning is a remarkably versatile method for creating seamless gene fusions. SnapGene was the first software to simulate this procedure. For the In-Fusion reaction, a linearized vector is mixed with one or more PCR products that have overlapping ends. SnapGene simplifies In-Fusion cloning by automating the primer design. To plan an In-Fusion reaction, just select the DNA fragments that you wish to fuse, and SnapGene will choose suitable primers. To learn more about in-fusion cloning visit the in-depth guide: https://www.snapgene.com/guides/in-fu... IN THIS VIDEO: 0:00 0:43 Setting Up the Experiment 1:06 Inserting the BDNF Gene Promoter 2:00 Selecting the Insert Fragment 2:34 Choosing PCR Primers 3:17 Previewing the Construct Visit snapgene.com to learn more. SnapGene Home: https://www.snapgene.com/ SnapGene Resources: https://www.snapgene.com/resources/ SnapGene Free Trial: https://www.snapgene.com/free-trial