How to Simulate Gateway Cloning in SnapGene
Plasmids can be constructed without restriction enzymes using Gateway Cloning, which inserts DNA fragments by recombination. SnapGene simulates different variations of this method. For standard Gateway cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an Entry Vector. Then the fragment is transferred to a Destination Vector in an LR cloning reaction, creating the final Expression Vector. Multisite Gateway cloning allows up to four fragments to be inserted simultaneously. To learn more about Gateway cloning check out the in-depth guide: https://www.snapgene.com/guides/gatew... IN THIS VIDEO: 00:00 01:14 BP Reaction 03:04 LR Reaction 04:15 Single-step reaction Visit snapgene.com to learn more. SnapGene Home: https://www.snapgene.com/ SnapGene Resources: https://www.snapgene.com/resources/ SnapGene Free Trial: https://www.snapgene.com/free-trial
Gateway cloning | How does Gateway cloning work? | What are the advantages of Gateway cloning? |
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