TP1-2 Biochimie : Détermination de la vitesse initiale d'une réaction enzymatique

The Enzymology and Metabolic Biochemistry module PDF SVI S4. Bachelor of Science in Life, Earth, and Universe Sciences. It includes lectures, summaries, tutorials, practicals, multiple-choice questions, exercises, solved exams, free books, and more… You can study with us online and take multiple-choice questions on our forum. You can also download the PDF files and study offline. In any case, you should return to this main page for easy navigation. Enzymology 1- Definition and classification of enzymes 2- Roles of enzymes a- The active site; enzymatic catalysis and the different types of catalysis. b- Properties of enzymes: Specificity and efficiency 3- Enzyme kinetics a- First-order kinetics (rate, etc.) b- Influence of substrate concentration on enzymatic activity 4- Enzyme effectors a. Inhibitors b. Activators 5- Physicochemical parameters a. Temperature b. pH Metabolic Biochemistry Content: Introduction and definition of metabolism Bioenergetics Definition, Free energy G, Enthalpy, Entropy, Bonds with high hydrolysis potential. Redox reactions and Redox potential. Concept of coupled reaction and common intermediate. Main molecules involved in redox reactions: electron carriers Carbohydrate Metabolism Glycolysis a. Aerobic: Krebs cycle, b. Anaerobic: fermentation) – Pentose phosphate pathway – Mechanism of Oxidative Phosphorylation – Regulation of glycolysis Lipid metabolism Lipid biosynthesis Lipid degradation Amino acid metabolism Introduction to amino acids Amino acid degradation Amino acid biosynthesis Other metabolic pathways (orogeny, etc.) Introduction In biochemistry, enzymology studies enzymes and fermentation. Several more specific areas can be identified: the study of enzymatic reactions, including reaction kinetics, the structural approach to enzymes and their relationship to their activity, which also allows for the classification of enzymes according to EC nomenclature. Fermentation, as well as anaerobic respiration, are thought to be older processes than aerobic respiration. The initially low oxygen levels on Earth prompted anaerobic mechanisms in the first organisms. Eukaryotes evolved aerobic respiration by developing the ability to use oxygen produced through photosynthesis. Biochemistry is the study of chemical reactions that occur within living organisms, particularly in cells. The complexity of biological chemical processes is controlled through cell signaling and energy transfers during metabolism.