FIJI (ImageJ): Estimation of Width Using Local Thickness

Learn how to use FIJI (ImageJ) to estimate the width, diameter or thickness of objects in 2D and 3D images or stacks via the Local Thickness plugin. Local thickness can be described as the diameter of the largest circle that fits inside the object and contains the point. This plugin involves an algorithm that first calculates a Euclidean distance map of foreground pixels. The distance map is then processed into a distance ridge, which simplifies local thickness computation. A local thickness map is generated, which can then be used to determine the diameter distribution of structures in an image. SUBSCRIBE to have first access to new video tutorials:    / @johanna.m.dela-cruz