RNA-seq tutorial with DESeq2: Differential gene expression project
Make your own bioinformatics project that reproduces a differential gene expression analysis using DESeq2 and the Gene Expression Atlas. Play with this data in Circa: https://circa.omgenomics.com/app/plot... Timestamps: 0:00 Intro 1:11 Where to find published RNA-seq data 2:25 Download data from the Gene Expression Atlas 4:24 Wrangle the data for DESeq2 7:49 Spot check the data 9:17 Run DESeq2 12:50 More complex design formulas 16:13 What does the ~ mean? 17:32 Compare your results to the Gene Expression Atlas 19:55 Make an MA plot and Volcano plot 21:09 Make a circos plot R script: https://github.com/omgenomics/youtube... How the DESeq design formula works: • DESeq2 Design Explained: Understanding Int... Circa circos plotting software: https://circa.omgenomics.com/?yt=deseq Try Circa for free on RNA-seq data: https://circa.omgenomics.com/app/plot...
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