Gelatin Zymography Tutorial 5: Quantifying MMP Activity with Image J and Normalizing Samples

Here we demonstrate how to quantify bands of MMP activity in a Gelatin Zymogram using Image J, and then normalize the MMP activity of samples to an MMP-2 standard in Excel. We also explain the rationale behind normalizing samples with an MMP standard, which is to account for the variations which normally occur from gel to gel. Links mentioned in the video: Image J download website - http://rsbweb.nih.gov/ij/download.html Protocol for Preparing Active MMP-2 Standards - http://protocol-place.com/assays/gela... Gelatin zymography is a useful technique for measuring the activity of MMPs in various samples. This video tutorial series aims to help scientists who wish to learn this technique do so in as little time as possible and with minimal troubleshooting. A full Gelatin Zymography protocol as well as other protocols related to Gelatin Zymography can be found at http://protocol-place.com/assays/gela... Here are all the videos in this series (so far): Gelatin Zymography Tutorial 1: Collecting and Storing Samples - - -    • Gelatin Zymography Tutorial 1: Collecting ...   Gelatin Zymography Tutorial 2: Sample and Reagent Preparation - - -    • Gelatin Zymography Tutorial 2: Sample and ...   Gelatin Zymography Tutorial 4: Identifying MMPs by Molecular Weight - - -    • Gelatin Zymography Tutorial 4: Identifying...   Gelatin Zymography Tutorial 5: Quantifying MMP Activity with Image J and Normalizing Samples - - -    • Gelatin Zymography Tutorial 5: Quantifying...   This series is still being produced, so subscribe to our channels for updates when new videos are released: http://www.youtube.com/subscribe_widg... You can help the Protocol Place grow by liking and sharing these videos with others! Thanks for watching! Youssef Farhat Protocol Place: http://protocol-place.com **Check out some of our other tutorials via the links below** Competitive ELISA Tutorials - - -    • Competitive ELISA Tutorial 1: How a Compet...   ELISA Tutorials - - -    • ELISA Tutorial 1: How a Direct, Indirect a...   Gelatin Zymography Tutorials - - -    • Gelatin Zymography Tutorial 1: Collecting ...