Gelatin Zymography Tutorial 4: Identifying MMPs by Molecular Weight

In this video, we demonstrate how to identify which MMPs are detected on a Gelatin Zymogram using a Molecular Weight Protein Ladder. Scanned images of the zymogram before staining are manipulated in PowerPoint in order to identify the molecular weight of the bands of MMP activity that can be observed in the stained zymogram. We also demonstrate how to manipulate the colors of the zymogram in order to visualize bands of MMP activity better. Links mentioned in the video: Image J download website - http://rsbweb.nih.gov/ij/download.html Gelatin Zymography Band Interpretation - http://protocol-place.com/assays/gela... Gelatin zymography is a useful technique for measuring the activity of MMPs in various samples. This video tutorial series aims to help scientists who wish to learn this technique do so in as little time as possible and with minimal troubleshooting. A full Gelatin Zymography protocol as well as other protocols related to Gelatin Zymography can be found at http://protocol-place.com/assays/gela... Here are all the videos in this series (so far): Gelatin Zymography Tutorial 1: Collecting and Storing Samples - - -    • Gelatin Zymography Tutorial 1: Collecting ...   Gelatin Zymography Tutorial 2: Sample and Reagent Preparation - - -    • Gelatin Zymography Tutorial 2: Sample and ...   Gelatin Zymography Tutorial 4: Identifying MMPs by Molecular Weight - - -    • Gelatin Zymography Tutorial 4: Identifying...   This series is still being produced, so subscribe to our channels for updates when new videos are released: http://www.youtube.com/subscribe_widg... You can help the Protocol Place grow by liking and sharing these videos with others! Thanks for watching! Youssef Farhat Protocol Place: http://protocol-place.com **Check out some of our other tutorials via the links below** Competitive ELISA Tutorials - - -    • Competitive ELISA Tutorial 1: How a Compet...   ELISA Tutorials - - -    • ELISA Tutorial 1: How a Direct, Indirect a...   Gelatin Zymography Tutorials - - -    • Gelatin Zymography Tutorial 1: Collecting ...