Webinar: Unbiased stereology to count cells in regions of interest

The Optical Fractionator is an unbiased stereological method that is used to accurately and efficiently estimate numbers of particles. Drs. Jose Maldonado and Dan Peruzzi will discuss the theory behind the Optical Fractionator and discuss its use. They will also give practical advice for planning an experiment using the Optical Fractionator so the desired precision is achieved. This theoretical framework will support the practical application of the Optical Fractionator in your laboratory's research. There are important rules to follow when carrying-out Optical Fractionator so that the estimate of the number of cells is unbiased. You will learn about these rules and how they work to prevent the over-sampling of cells. We will also talk about how much counting you need to do in order to get an estimate that is precise enough for your needs while avoiding doing extra work and wasting the investigators time. This seminar will not actually go over the steps for performing an Optical Fractionator in Stereo Investigator; but it will make that process much easier to learn.