Webinar: Introduction to Stereo Investigator

Stereo Investigator provides you with accurate, unbiased estimates of the number, length, area, and volume of cells or biological structures in a tissue specimen. It is a key research tool that has helped lead advances in numerous areas of neuroscience. For more information on Stereo Investigator: https://www.mbfbioscience.com/stereo-... To request a free demonstration of Stereo Investigator: https://www.mbfbioscience.com/request... 0:00 - Intro 0:29 - Introduction to the Webinar 0:52 - Overview of Topics 2:39 - Bias in Stereology 5:07 - Precision and Accuracy 7:39 - What Can Stereology Do For You? 8:23 - Hardware Integration 9:47 - Opening and Using the Program 10:49 - Troubleshooting the Brightfield Light Path 12:20 - Checking the Software 13:16 - Brief Introduction of Interface 15:46 - Optical Fractionator Workflow 19:32 - Oil Objective (Objective Lens Importance) 21:21 - Measure Mounted Thickness 21:48 - Counting Frame Size 22:33 - SRS Grid Layout 25:50 - Dissector Options 27:29 - Save Sampling Parameters 27:47 - Count Objects 28:46 - Counting Cells Criteria 29:50 - Marking Cells 32:12 - Results 34:31 - Optical Fractionator Questions 36:41 - Cavalieri 42:49 - Image Acquisition & Slide Scanning 51:16 - Questions 54:28 - MBF Resources 55:02 - Outro