ELISA Tutorial 2: Coating and Blocking the ELISA Plate

Here, we illustrate the process and concept behind the initial steps of a Sandwich ELISA: coating an ELISA plate with a capture antibody and then blocking the plate with BSA or a detergent-based Blocking Buffer. Our ELISA tutorials are kindly sponsored by Calbiotech. Click here to see Calbiotech's ELISA kits: http://protocol-place.com/Calbiotech This video and other protocols can be found at our website, the "Protocol Place" - http://protocol-place.com/ A full ELISA protocol can be found at http://protocol-place.com/assays/sand... This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series: ELISA Tutorial 1: Understand How an ELISA Works -    • ELISA Tutorial 1: How a Direct, Indirect a...   ELISA Tutorial 2: Coating and Blocking the ELISA Plate -    • ELISA Tutorial 2: Coating and Blocking the...   ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate -    • ELISA Tutorial 3: Preparing and Adding Sam...   ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) -    • ELISA Tutorial 4: Finishing the Assay (San...   ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism -    • ELISA Tutorial 5: Preparing ELISA Data in ...   ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism -    • ELISA Tutorial 6: How to Analyze ELISA Dat...   Competitive ELISA Tutorial 1: How a Competitive ELISA Works -    • Competitive ELISA Tutorial 1: How a Compet...   Competitive ELISA Tutorial 2: How to Use Calbiotech's Competitive ELISA Kits -    • Competitive ELISA Tutorial 2: How to Use C...   Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel -    • Competitive ELISA Tutorial 3: Analyzing Ty...   We hope you enjoy watching and benefit from our tutorials. If so, please take a minute to "like," or better yet, share them with others! Thanks for watching! Youssef Farhat Protocol Place: http://protocol-place.com **Check out some of our other tutorials via the links below** Competitive ELISA Tutorials - - -    • Competitive ELISA Tutorial 1: How a Compet...   ELISA Tutorials - - -    • ELISA Tutorial 1: How a Direct, Indirect a...   Gelatin Zymography Tutorials - - -    • Gelatin Zymography Tutorial 1: Collecting ...