Validating Protein Interactions with co-IP Using Endogenous and Tagged Protein Models

Immunoprecipitation (IP) leverages the specificity of an antibody to enrich proteins in a sample and investigate its interactions with other macromolecules. This presentation includes a general workflow for co-immunoprecipitation (co-IP) and what you should consider before setting up your experiment, including: – Choice of model system; – Choice of cell lysis buffers and when to include a pre-clear step; – Primary antibody dilution and choice of beads; – How to mitigate IgG masking by heavy and light chains; – Using epitope tags for IP: advantages and key optimization considerations; – Controls for IP experiments targeting post-translational modifications. For more expert guidance on setting up your IP experiment, watch this Tech Tips video: How to Select an Antibody for Immunoprecipitation    • How to Select an Antibody for Protein Immu...   For technical support to get your experiments working, visit https://cellsignal.com/support About CST®: Cell Signaling Technology (CST) is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools to the biomedical research community. Our employees operate worldwide from our U.S. headquarters in Massachusetts, and our offices in the Netherlands, China, and Japan. https://cellsignal.com/about All trademarks are the property of their respective owners. For the most up-to-date trademark information, please visit https://www.cellsignal.com/trademarks #CSTLearningLabLive #antibody #immunoprecipitation