No More (Data) Secrets: Unlock the Molecular Mechanisms of Pathogenic and Non PathogenicTh17 Cells

Single-cell analysis (SCA) continues to increase in popularity due to technological advancements and its ability to identify hard-to-detect cells, reveal cellular heterogeneity, and show how individual cells interact with their environments. While most SCA techniques detect RNA and surface proteins, they are incapable of concurrently measuring intracellular proteins and post-translational modifications, resulting in a truncated view of cellular processes and disease mechanisms. To unlock biological insights more efficiently, researchers “seq” to extract more comprehensive data from the same experiment without additional experiments or validation of results. InTraSeq™ Single Cell Analysis developed by CST enables co-quantification of RNA, both surface and intracellular proteins, and post-translational modifications at a single-cell resolution—in one experiment. Integrating transcriptomic, proteomic, and intracellular interaction data enables more comprehensive analysis to scale our understanding of cellular processes. Learn how InTraSeq empowers single-cell and translational researchers to: ∙Detect RNA, intracellular, and cell surface proteins in thousands of cells simultaneously while guaranteeing robust RNA signal. ∙Investigate intracellular signaling pathways together with the transcriptome at the single-cell resolution. ∙Identify hard-to-detect cells with an unbiased depth of coverage. Explore molecular mechanisms of pathogenic and non-pathogenic Th17 cells in one, streamlined experiment. For more, visit the InTraSeq Single Cell Analysis Resource Center: https://www.cellsignal.com/applicatio... Contents: 0:00 Introduction 2:16 Understanding Single Cell Analysis 3:24 Single cell RNA-seq reveals the heterogeneity of the sample based on transcriptomic data 6:37 Challenges in Single Cell Assays 7:50 What is InTraSeq technology? 10:04 Data Insights from InTraSeq Experiments 11:56 Correlation Between RNA and Protein Levels 13:27 Analyzing T Cell Differentiation States 15:25 Measuring Post-Translational Modifications 17:41 Comprehensive Understanding of Stat3 Signaling 20:02 InTraSeq vs Western Blot readout reliability 22:37 Time-Dependent Changes in T Cell Stimulation 29:40 Comparative Analysis of Non-Pathogenic and Pathogenic T Cells 32:25 Identifying Mitotically Active Cells with Phospho-Histone Antibody 34:47 Multiplexing Samples with InTraSeq 38:41 Surface vs Intracellular Hashing 41:50 CST Custom Conjugation Service for InTraSeq 43:38 Q&A About us: Cell Signaling Technology® (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality, technological innovation, and scientific rigor. We are a company of caring people driven by a devotion to facilitating good science—a company committed to doing the right thing for our Customers, our communities, and our planet. https://cellsignal.com/about Cell Signaling Technology, CST, and InTraSeq are trademarks of Cell Signaling Technology, Inc. All rights reserved. 10x Genomics, 10x, Feature Barcode, and Chromium are the trademarks or registered trademarks of 10x Genomics, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information. Subject to patents licensed from 10x Genomics, Inc., for use with single-cell (i.e., Chromium) 10x products.