2- HPLC KROMATOGRAFİ KULLANIM EĞİTİMİ/KROMATOGRAFİ TEORİSİ(THEORY OF CHROMATOGRAPHY )

High Performance Liquid Chromatography Training, High Performance Liquid Chromatography Training. Lab Akademi Webinar HPLC System Overview Seminar. Shimadzu, Waters, Thermo, Agilent, Empower, Chromalien, Chemstation A. ACCORDING TO THE TYPE OF MOVING PHASE; 1. Liquid Chromatography (LC) liquid-solid liquid - liquid 2. Gas Chromatography (GC) gas-solid gas-liquid 3. Supercritical Fluid Chromatography (SFC) It is a type of chromatography in which substances (CO2) at critical temperature and pressure are used. B) ACCORDING TO THE APPLICATION FORM; paper chromatography Thin layer chromatography (TLC) Column (column) chromatography (CC) Gas chromatography (GC) 5) High performance liquid chromatography (HPLC) C. ACCORDING TO THE DISCRIMINATION MECHANISMS; 1. Adsorption chromatography 2. Partition chromatography 3. Ion exchange chromatography 4. Molecular elimination chromatography (Gel chromatography) 5. Affinity chromatography (Chemical chromatography) Types of chromatography column chromatography Ion-exchange chromatography Gel-permeation (molecular sieve) chromatography affinity chromatography paper chromatography Thin-layer chromatography Gas chromatography Dye-ligand chromatography Hydrophobic interaction chromatography pseudoaffinity chromatography High-pressure liquid chromatography (HPLC) For Upcoming Webinars: https://labakademi.com/yaklasan-web-s... To follow on social media; Instagram:   / .  . Twitter:   / labakademi   Facebook:   / labakademi   Linkedin:   / lab-a.  . Website: https://labakademi.com/ Youtube:    / labakademi   High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent which is known as the mobile phase at high pressure through a column with chromatographic packing material known as stationary phase. The sample is carried by a moving carrier gas stream of helium or nitrogen. HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion. Because of this versatility, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals. Sample retention time will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the solvent, or solvents used. As the sample passes through the column it interacts between the two phases at different rate, primarily due to different polarities in the analytes. Analyzes that have the least amount of interaction with the stationary phase or the most amount of interaction with the mobile phase will exit the column faster. This lecture explains the following things about Hplc chromatography - 1. Hplc chromatography principle 2. Hplc chromatography instrumentation 3. Hplc chromatography types High-Performance Liquid Chromatography - Other HPLC Types Ultra High Performance Liquid Chromatography (uHPLC): Where standard HPLC typically uses column particles with sizes from 3 to 5µm and pressures of around 400 bar, uHPLC use specially designed columns with particles down to 1.7µm in size, at pressures in excess of 1000 bar. The main advantage of an uHPLC is speed. These systems are faster, more sensitive, and rely on smaller volumes of organic solvents than standard HPLC, resulting in the ability to run more samples in less time. Lab Academy organizes laboratory, science, device, analysis, training, seminar, free training, live, online, on-line, webinar, webinar, training. You can watch these trainings on the training platform and on our Youtube channel. Our trainings are fun, understandable, simple, academic, theoretical, practical, experience-based, free, live, online, web-based content.