HPLC SİSTEMİNE MOBİL FAZ YÜKLEME İŞLEMLERİ #HPLC #kromatografi

#labakademi #hplc #chromatography #chromatography #vrwhitachi #hitachi #analytical #laboratory #terraas This video was shot in the Lab Academy Experience and Training Center laboratory under the sponsorship of Terra A.Ş., the sole authorized distributor of VWR Hitachi Turkey. We would like to thank TERRA Analiz ve Ölçüm Cihazları Tic. A.Ş. for sponsoring our video. If you are in the process of purchasing an HPLC system or among your plans, you can contact Terra AŞ from the contact information below to request a demo and experience the privileges of the VWR Hitachi HPLC system. Web Site: https://www.terraanaliz.com.tr/iletisim Mail: [email protected] For Face-to-Face and Hands-on HPLC Training: https://labakademi.com.tr/hplc-egitimi-2 Here are some important points to consider during the mobile phase loading process for HPLC system: Mobile Phase Selection: It is important to select the appropriate mobile phase for the analysis. The mobile phase should be selected according to the polarity, solubility and separation ability of the compounds analyzed. It is also important to choose a mobile phase that matches the characteristics of the system. Preparation of the Mobile Phase: It is important to prepare the mobile phase correctly. A good mobile phase preparation is essential to obtain a homogeneous mixture. The mobile phase should be prepared using solvents in the correct proportions and of appropriate quality. Filtration: Filtration of the prepared mobile phase is important to prevent particulate or solid residues from entering the system. This prevents blockages in the column and system and makes the analysis results accurate and reproducible. Monitoring Variables: During the mobile phase loading process, it is important to continuously monitor variables such as pressure, temperature and flow rate. This helps to optimize system performance and ensure the accuracy of analyses. Calibration and Adjustment of Variables: Components used in the HPLC system such as the mobile phase pump, injector and detector need to be calibrated and adjusted regularly. This ensures accurate and reproducible analysis results. Compliance with Instructions for Use: Each HPLC system can be different, so it is important to follow the manufacturer's instructions during the mobile phase loading process. Failure to follow the instructions for use can affect the performance of the system and make analysis results misleading. Avoiding Contamination: Contamination should be avoided during the mobile phase loading process. Care must be taken to ensure that the glassware used is clean and to reduce the risk of cross-contamination. Control of Environmental Factors: Controlling laboratory conditions (temperature, humidity, etc.) increases the reliability of mobile phase loading and analysis. To follow on social media; Instagram:   / labakademi   Twitter:   / labakademi   Facebook:   / labakademi   Linkedin:   / lab-akademi   Website: https://labakademi.com/ Youtube:    / labakademi   Translated with DeepL.com (free version)