Digestible and Resistant Starch Assay Kit with K-DSTRS

Technical Video Protocol for Megazyme's Digestible Starch & Resistant Starch Assay (K-DSTRS) A procedure for the specific measurement and analysis of digestible and resistant components of starch with a single kit. ** Subtitles are available, but must be manually turned on and can be changed to your desired language ** 00:05 Introduction 01:42 Principle 04:11 Reagent Preparation 07:54 Enzyme Digestion: Method 1 – Analysing ~ 0.5 g of sample 09:21 Method 1 (A) – Addition of PAA + AMG solution 09:50 Method 1 (B) – Addition of PAA + AMG suspension 10:23 Enzyme Digestion: Method 2 – Analysing ~ 1.0 g of sample 11:34 Method 2 (A) – Addition of PAA + AMG solution 12:03 Method 2 (B) – Addition of PAA + AMG suspension 12:33 Preparation of Stopping Reagents 13:12 Determination of Digestible Starch 18:27 Determination of Resistant Starch 25:53 Calculations This video will talk you through the assay principle, procedure, and will also explain how to perform the necessary calculations. • Product page at the Megazyme webstore: https://www.megazyme.com/digestible-a... • Download the K-DSTRS data booklet: https://support.megazyme.com/support/... • Access the Mega-Calc for K-DSTRS: https://support.megazyme.com/support/...