Determinación espectrofotométrica de clorofila: Método tricromático | | UPV

Title: Spectrophotometric Determination of Chlorophyll: Trichromatic Method Description: Determination of photosynthetic pigments (chlorophylls) in water samples using the trichromatic method. Pachés Giner, MAV; Martínez Guijarro, MR. (2015). Spectrophotometric Determination of Chlorophyll: Trichromatic Method. http://hdl.handle.net/10251/53651 Automatic description: This video explains how to determine the chlorophyll concentration in water samples using the YQ method, which allows the identification of different types of chlorophyll, crucial pigments for photosynthesis in cells of phototrophic organisms such as plants and algae. The process begins with filtration to remove particulate matter, and then chlorophyll is extracted from the material retained in the filter for analysis by spectrophotometry. The materials required for each step are listed, including membrane filters, a vacuum pump filtration system, and 90% acetone for extraction. The procedure details the steps from sample filtration and freezing for preservation and cell disruption to pigment extraction and separation by centrifugation. Finally, the sample's absorbance is measured at different wavelengths, including a value to account for non-pigment absorbance, and formulas are applied to calculate chlorophyll 'a' and 'b' concentrations. The results demonstrate the practical application of the equations for obtaining the concentrations. Author: Paches Giner Maria Aguas Vivas Polytechnic University of Valencia UPV: https://www.upv.es More videos at:    / valenciaupv   Access our MOOCs: https://upvx.es #Water samples #Chlorophyll #Spectrophotometer #ENVIRONMENTAL TECHNOLOGY