Mastering mRNA-based Therapeutics Analysis | Biopharma 101 by SCIEX

The incredible success of #mrna #vaccines against COVID-19 has generated a surge of interest and investment in the development of this new therapeutic modality. Products based on mRNA have broad potential for use as therapeutics in applications such as cancer treatments and vaccines. In addition, there are many advantages to using mRNA over existing technologies, including reduced risk of the unwanted post-translational modifications that can plague protein therapeutics. However, the pursuit of new therapeutic modalities can raise unforeseen risk during research and development. 𝗕𝗶𝗼𝗽𝗵𝗮𝗿𝗺𝗮 𝟭𝟬𝟭 𝗯𝘆 𝗦𝗖𝗜𝗘𝗫 is intended to provide you with a thorough introduction to foundational analytical characterization workflows for mRNA-based therapeutics, from the basics of mRNA chemistry and an overview of manufacturing. This foundation can then be applied to the use of capillary electrophoresis for plasmid and mRNA purity profiling, as well as the use of mass spectrometry to characterize the specific type of 5’-cap and identify lipid nanoparticle impurities; attending this class will upgrade your mRNA therapeutic expertise! 𝗟𝗲𝗮𝗿𝗻𝗶𝗻𝗴 𝗢𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲𝘀 ▷ Quantify plasmid topological forms to ensure starting material quality ▷ Determine mRNA purity and sequence length with a reliable size estimation ▷ Elucidate RNA structural modeling by chemical probing and sequencing ▷ Reliably identify the different mRNA capping structures as well as distinguish between uncapped and capped structures. ▷ Determine drug efficacy and safety by identifying oxidative impurities in lipid nano particle formulations 𝗣𝗿𝗲𝘀𝗲𝗻𝘁𝗲𝗿𝘀 🎤 Paula Orens, Market Development Manager, Biopharma MS, SCIEX 🎤 Steven Calciano, Market Development Manager, Biopharma CE, SCIEX 🎤 Nathan Kondor, Workflow Specialist, Nucleic Acid Therapies, Cytiva