Principles of surface plasmon resonance (SPR) used in Biacore™ systems - Cytiva

The surface plasmon resonance (SPR) technology in Biacore systems detect and quantify binding between two molecules in an automated and highly sensitive process. As the SPR detection principle relies on changes in mass, and allows you to analyze interactions in real-time without the need for labels. The technology provides binding kinetics, affinity, specificity, concentration, epitope binning and more. This is true for any size of molecule in a pure or complex sample. Discover Biacore™ SPR : https://cytiva.link/eb0vu Learn more about SPR: https://www.cytivalifesciences.com/en... Chapters: 00:03 What is Surface Plasmon Resonance (SPR)? 00:22 What SPR Measures: Affinity, Kinetics, Concentration, Specificity 00:44 Core Components of Biacore Systems 01:05 Microfluidics, Buffers, and Software Control 01:24 Sensor Chip Structure and Surface Chemistry 01:58 Immobilizing the Ligand on the Sensor Chip 02:18 Injecting the Analyte and Running the Experiment 02:38 How SPR Detection Works 03:16 Understanding the Sensorgram Output 03:40 SPR Data: Affinity, Kinetics and Specificity 03:59 Label-Free Interaction Analysis Benefits Stay informed with the latest from Cytiva. Subscribe to our YouTube channel:    / @cytiva   #SurfacePlasmonResonance #Biacore #BiomolecularInteractions