Tn-seq in Rhodobacter sphaeroides - mSystems®

Knowledge about the role of genes under a particular growth condition is required for a holistic understanding of a bacterial cell and has implications for health, agriculture, and biotechnology. We developed the Tn-seq analysis software (TSAS) package to provide a flexible and statistically rigorous workflow for the high-throughput analysis of insertion mutant libraries, advanced the knowledge of gene essentiality in R. sphaeroides, and illustrated how Tn-seq data can be used to more accurately identify genes that play important roles in metabolism and other processes that are essential for cellular survival. Combining Genome-Scale Experimental and Computational Methods To Identify Essential Genes in Rhodobacter sphaeroides Brian T. Burger, Saheed Imam, Matthew J. Scarborough, Daniel R. Noguera, Timothy J. Donohue (http://orcid.org/0000-0001-8738-2467) Stephen R. Lindemann, Editor Published in mSystems® on 6 June 2017 Direct link: https://doi.org/10.1128/mSystems.0001... mSystems® publishes preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems® encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems® welcomes submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems® provides streamlined decisions, while carrying on ASM's tradition of rigorous peer review. Subscribe to ASM's YouTube channel at https://goo.gl/mOVHlK Learn more about the American Society for Microbiology at http://www.asm.org Become a member today at http://www.asmscience.org/join Interact with us on social at: Facebook Show your support and get updates on the latest microbial offerings and news from the ASM.   / asmfan   ASM International Facebook Groups Join an ASM International Facebook Group and connect with microbiologists in your region. http://www.asm.org/index.php/programs... Twitter Follow all the latest news from the Society.   / asmicrobiology   Instagram Outstanding images of your favorite viruses, fungi, bacteria and parasites   / asmicrobiology