Best Practices and Material Requirements | BCA Workflow

These best practices are helpful to remember when preparing and running your experiment, as well as when you are creating your standard curve. Don't forget to run your samples in triplicate. Also, remember to never extrapolate data from your standard curve. Each vendor's BCA kit could be slightly different, so be sure to check with the vendor's recommendations before running your protein assay. BCA Assay Kit For more information about the BCA assay kit mentioned in this course, review the following data sheet from Thermo Scientific. https://www.thermofisher.com/document... About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://shop.licorbio.com/reagents/ Image Studio™ Software https://www.licorbio.com/image-studio LinkedIn   / licorbio   Twitter   / licorbio   Facebook   / licorbio