Colony PCR Protocol (BIOL310)

In this video, I walk you through the procedure of screening bacterial colonies for your gene of interest using Colony PCR. In the process, I also introduce the concept of a PCR Master Mix, which will simplify and speed up your work. 0:00 - Introduction 0:40 - Preparing bacterial samples for use as template DNA 2:26 - Preparation of a PCR Master Mix 5:20 - Completing the PCR reaction setup