Biological Mass Spectrometry and Proteomics - J. Mark Shekel

The LMB Mass Spectrometry and Proteomics Facility houses a wide range of mass spectrometers enabling biological proteomics and structural proteomics. Biological proteomics involves the identification of proteins and their interactions and the comparative proteomics of cells and tissues. In structural proteomics purified proteins or their complexes are investigated by hydrogen-deuterium exchange or cross-linking mass spectrometry. In this video Mark begins with a brief history of mass spectrometry and how samples are ionized and measured by the mass spectrometer. He then follows with a detailed explanation of mass-spectrometry approaches to biological proteomics via protein identification of single proteins and their interactions in cells via pull-down assay or proximity dependent biotin identification. In the last part of the talks the discusses different methods for quantitative proteomics via label-free, isotopic labelling or reporter ion-based quantitation. Finally he shows how post-translational modifications can be investigated. The Mass Spectrometry and Proteomics Facility is headed by J Mark Skehel. Read more about the facility here: https://www2.mrc-lmb.cam.ac.uk/resear... You can find more videos from the LMB 2021 Biophysics Techniques Lecture series here:    • Introduction to Biophysical Techniques 2021   Other playlists you might be interested in: Next Generation Biophysics Symposium 2020 -    • Next Generation Biophysics Symposium 2020   Electron Cryo-Microscopy Course 2017 -    • Electron Cryo-microscopy Course 2017   RNA Seq Course -    • RNA-seq Course 2020   LMB Crystallography Course 2013 -    • LMB Crystallography Course 2013   -- About the MRC Laboratory of Molecular Biology (LMB): The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Its scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health. More links: Official Site: https://www2.mrc-lmb.cam.ac.uk/ Facebook:   / mrc.lmb   Twitter:   / mrc_lmb   Instagram:   / lmbnews   LinkedIn:   / mrc-laboratory-of-molecular-biology   Click here to subscribe to MRC Laboratory of Molecular Biology on YouTube: https://www.youtube.com/user/LMBCambr...