Arndt Asperger, Ph.D., Senior Applications Scientist, Bruker Daltonics GmbH & Co. KG

Bruker @ SLAS 2022 – Now On-Demand Title: timsTOF MALDI PharmaPulse: A next-generation MS based label-free uHTS solution taking advantage of timsTOF technology Mass spectrometry (MS) offers huge potential for enhancing the efficiency of early drug discovery. In this workshop, we will introduce the timsTOF MALDI PharmaPulse (MPP), a next-generation label-free solution for MS based HTS and uHTS taking advantage of Bruker´s innovative timsTOF technology. timsTOF MPP combines proven speed and robustness of MALDI with trapped ion mobility (TIMS) separation and high-resolution, accurate-mass time-of-flight (TOF) MS detection, allowing for new, deeper insights into molecular interactions at uHTS compatible reading rates. Advanced timsTOF MPP operation modes provide enhanced assay specificity and thus, open up exciting new HTS possibilities. Analysis in MS/MS mode utilizes compound-specific fragment ions resulting in expanded range of quantitation. TIMS offers an additional dimension of gas-phase separation for efficient reduction of isobaric or isomeric background interferences running significantly faster than any LC/SPE based separation method currently available. Key features described above, make the timsTOF MPP a highly versatile platform for a broad range of HTS and uHTS applications in early drug discovery, including biochemical and cell-based mechanistic assays, cell-uptake assays and binding assays. In addition, timsTOF MPP serves as a highly capable platform for near real-time verification of synthesis products obtained from high-throughput experimentation (HTE) chemistry in search of new drug molecules. timsTOF MPP solutions includes dedicated MALDI PharmaPulse HTS software, guiding the user through setup and execution of various screening workflows, featuring an automation interface for seamless integration with lab automation systems and providing a customizable export interface for transfer of data and results to external downstream analysis software.