A Brief Guide to Metagenomic Sequence Analysis
Dr. Nastassia Patin, a postdoctoral associate with the National Oceanic and Atmospheric Administration’s Atlantic and Oceanographic Meteorological Laboratory (NOAA AOML), provides a presentation on the basics of analyzing shotgun metagenomes, specifically short read metagenomes. Nastassia Patin can be contacted at [email protected]. Timestamps: 00:00 - Introduction 00:51 - General workflow 02:02 - 01. Read quality control 04:15 - 02. Read-based analyses 09:28 - 03. Assembly 11:48 - 04. Gene prediction 13:00 - 05. Gene annotation 17:35 - 06. Coverage 19:35 - 07. Binning 23:53 - 08. Genome quality 25:12 - 09. Genome taxonomy 26:17 - 10. Dereplicating MAGs 29:28 - Summary 32:02 - Pan-metagenome thought exercise
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