Determination of pI (Isoelectric Point) of Diprotic Amino Acids | pK₁, pK₂ & Titration Curve

In this video, I explain how to determine the isoelectric point (pI) of diprotic amino acids using pK₁ and pK₂ values. The lecture covers the concept of zwitterions, potentiometric titration, titration curves, and the laboratory method used to calculate pI. Topics Covered: • What is pI (Isoelectric Point)? • Diprotic Amino Acids • pK₁ and pK₂ Values • Zwitterionic Form • Potentiometric Titration Method • Titration Curve Analysis • Calculation of pI with Examples • Important CSIR NET and GATE Questions This lecture is useful for CSIR NET Life Sciences, GATE Biotechnology, SET, MSc Entrance, and university examinations. #IsoelectricPoint #pI #AminoAcids #DiproticAminoAcids #CSIRNETLifeSciences #Biochemistry #ProteinChemistry #GATEBiotechnology #LifeSciences #biologylecture By Sushma Agrawal