From cryo-EM data to continuous conformational landscapes - Slavica Jonic - Shape seminar

10th session of the shape seminar in Paris - 7 February 2024. Slavica Jonic (CNRS & Sorbonne Université) presents an overview of modern methods to reconstruct manifolds of 3D protein poses from 2D cryo-electron microscopy images. For links to the slides and information about future events: https://shape-analysis.github.io/ Introduction. 0:00 Cryo-EM. 6:36 Discrete-classification approaches. 24:02 Dealing with continuous conformational manifolds. 35:37 Methods. 45:07 Pros and cons. 52:56 Simulating conformational dynamics. 59:54 Sampling with molecular dynamics. 1:04:45 Sampling with normal mode analysis. 1:14:39 Overview and results. 1:22:19 Cryo-Electron Tomography. 1:32:01 Methods. 1:39:00 Conclusion. 1:40:44