Estimation of Serum Total Cholesterol by Enzymatic (CHOD-POD) Method || Biochemistry Practical
Estimation of Serum Total Cholesterol by Enzymatic (CHOD-POD) Method - This is the video on estimation of serum total cholesterol by cholesterol oxidase and peroxidase method with discussion of viva questions and demonstration. Aim and Introduction - 00:30 minutes Importance of estimation of cholesterol - 01:05 minutes Important biological compounds derived from cholesterol - 01:48 minutes Principle of Cholesterol Estimation by Enzymatic Method - 02:22 minutes Operational Procedure - 03:11 minutes Calculation of Total Cholesterol - 03:55 minutes Normal Range of Total Cholesterol - 04:38 minutes Lipid Profile - 04:43 minutes Friedewald equation - 05:15 minutes Hypercholesterolemia - 07:25 minutes Hypocholesterolemia - 07:51 minutes Viva Questions - 08:11 minutes Demonstration of Estimation of Serum Total Cholesterol by Enzymatic (CHOD-POD) Method - 11:17 minutes Cholesterol is the most abundant steroid in the human tissue and body fluids. It is synthesized in the liver and transported in the blood mainly in the form of LDL and HDL. In blood, cholesterol is present in two forms: Free (20 - 25%) and Esterified (75 – 80%). Important compounds derived from cholesterol: Bile acid 1ry bile acid- Cholic acid & Chenodeoxy cholic acid 2ndry acid- Lithocholic acid & Deoxycholic acid 2. Vit.D 3. Steroid hormones Subscribe my channel from - / biochemistrybasicsbydramit Follow me On Twitter - / dr_biochemistry On Facebook - / amit.v.m
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