Hexokinase Vs Glucokinase | Regulation of Glycolysis | Metabolism | Biochemistry Basics

This video provides a tutorial on important difference between hexokinase and glucokinase enzyme. Hexokinase enzyme has low Km (0.1 mM, high affinity for substrate) and low Vmax) low-capacity enzyme). This allows hexokinase enzyme to convert glucose to glucose-6-phosphate even under the presence of low glucose concentration. On the other hand, glucokinase enzyme Vmax (high-capacity enzyme). This allows glucokinase enzyme to convert glucose to glucose-6-phosphate only in the presence of plenty of tissue glucose concentration, for instance after carbohydrate-rich meal. The following topics are covered in this lesson, 1. Hexokinase enzyme 2. Glucokinase enzyme 3. Difference between hexokinase and glucokinase enzyme 4. Km of hexokinase enzyme 5. Vmax of hexokinase enzyme 6. Km of glucokinase enzyme 7. Vmax of glucokinase enzyme 8. Importance of glucokinase and hexokinase in regulating blood glucose level 9. Glucokinase Vs hexokinase affinity for glucose 10. Glucokinase Vs hexokinase function 11. Glucokinase Vs hexokinase regulation