Next Generation Sequencing Techniques and their applications
As Next Generation Sequencing becomes a first stop for most analysis downstream - DNASeq, RNASeq, ChIPSeq, ATACSeq, FARESeq .... etc, here is an elaborate take on the evolution of sequencing techniques - from the first to second to the third Generation, from intermediate read length (Sanger) to short reads - Illumina and Ion Torrent, to finally long read sequencing - Nanopore and SMRT, from sequencing the first human genome in about 10 years to sequencing it in less than a day, from sequencing it for 3 billion dollars to less than a 1000 dollars - Be my guest on this journey most remarkable - This is a recorded webinar of my presentation on 'Next Generation Sequencing Techniques and their applications' at Hindu College, University of Delhi #NGS #Genomics #Sequencing #Nanopore #Illumina #Ion_Torrent #SMRT #Short_reads #Long_reads_sequencing #Genome #Next_Generation_Sequencing #lets_grow_together #vipins_e_classroom 0:00 Introduction 1:34 Genome Sequencing steps 5:01 Depth / Coverage 7:28 Polymerase based sequencing 9:38 Automated Sanger Sequencing 12:18 The evolution of Sequencing technologies 14:04 Contemporary Sequencing technologies 14:41 COMMON FEATURES OF II GENERATION SEQUEN 16:27 Virtual Terminator sequencing-Illumina, Sole 21:42 lon torrent 27:27 SMRT - Single Molecule Real Time Sequencing 29:11 Nanopore - Helicase based sequencing 30:02 Biological Nanopore 30:17 Nanopore - principle 31:03 Oxford Nanopore 31:59 Nanopore - advantages 32:28 The Game changer - ultra long read 34:16 NGS Broad Applications 35:18 Key features of NGS 36:22 Exome Sequencing 37:52 NGS based diagnostics - Targeted gene sequencing 41:13 Metagenomics - 16S RNA typing 41:42 Microbiome project 42:43 Pharmacogenomics 42:59 Purine Analogs: A Case Study in Pharmacogenetics 44:41 TPMT Variants and effect on drug dosage 48:00 Genome Sequencing Milestones 48:58 Largest genome ever sequenced 50:04 Short read aligners 50:45 References 51:05 Desired skill set
ORF Finder - NCBI - predicting potential protein coding regions - Bioinformatics Practical 4
Next Generation Sequencing 1: Overview - Eric Chow (UCSF)
How AI Cracked the Protein Folding Code and Won a Nobel Prize
StatQuest: A gentle introduction to RNA-seq
Next-Generation Sequencing Technologies - Elaine Mardis (2012)
Next Generation Sequencing 4: Checking Nucleic Acids with an Agilent BioAnalyzer - Eric Chow (UCSF)
How CRISPR lets us edit our DNA | Jennifer Doudna
Illumina sequencing | DNA sequencing by synthesis
NGS 1- Basic Concepts in Next Generation Sequencing
Next Generation DNA Sequencing
Introduction to Next Generation Sequencing and Population Genomics - Arushi Batra
Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)
Nanopore sequencing - sample prep and analysis
NGS Data Analysis 101: RNA-Seq, WGS, and more - #ResearchersAtWork Webinar Series
DNA sequencing methods
Overview of Illumina Sequencing by Synthesis Workflow | Standard SBS chemistry
The Successor to CRISPR May Be Even More World Changing
Single cell RNA sequencing overview | ScRNA seq vs Bulk seq | chemistry of ScRNA seq |Bio Techniques
Single Cell Sequencing - Eric Chow (UCSF)
