Purify & Isolate Your Compounds Using Flash & Preparative HPLC | Dr. Sambhav Kumbhani | CSI
A flash liquid chromatography purification system, which is preparative liquid chromatography (PLC) was used in a semi-preparative scale for the isolation and purification of oligostilbenes from the methanol extracts of Dipterocarpus semivestitus leaves. A method for the optimum chromatographic condition was developed on an ultra-high performance liquid chromatography (UHPLC) system. The method was transferred to the PLC with up-scaling modifications. Two stilbene trimers ampelopsin C, and -viniferin were isolated from the fractions. The purities of the compounds were determined by the UHPLC. #Flash_HPLC #Preparative_HPLC #UHPLC Please stay connected with CSI using our: Website: https://www.chromsocindia.org YouTube: / @chromatographicsocietyofin719 Facebook: / chromsocindia LinkedIn: / chromatographic-society-of-india
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